Journal: STAR Protocols

Article Title: Spatial analysis of transcript and protein levels in skeletal muscle

doi: 10.1016/j.xpro.2024.103378

Figure Lengend Snippet:

Article Snippet: SAPPHIRE PCR 8-tube strips, 0.2 mL, PP, blue, without cap, 125 pieces/box , Greiner Bio-One , 673274.

Techniques: Recombinant, Hybridization, Amplification, Isolation, Membrane, Software, Imaging, Microscopy, Sterility, Stripping Membranes

Journal: Phytopathology®

Article Title: Highly Sensitive and Rapid Detection of Citrus Huanglongbing Pathogen (‘Candidatus Liberibacter asiaticus’) Using Cas12a-Based Methods

doi: 10.1094/phyto-09-20-0443-r

Figure Lengend Snippet: Fig. 4. Detection of ‘Candidatus Liberibacter asiaticus’ (CLas) nrdB DNA with Cas12a alone, DNA endonuclease-targeted CRISPR trans reporter (DETECTR), and quantitative PCR (qPCR). A, Sensitivity of DETECTR versus Cas12a without recombinase polymerase amplification. Note that the CLas DETECTR assay enables robust detection of CLas nucleic acids at attomolar sensitivity. Error bars represent mean ± SD when n = 3 replicates. B, Sensitivity of real-time qPCR for nrdB DNA detection. Error bars represent mean ± SD when n = 3 replicates. qPCR experiments were performed three times with similar results. NC, negative control; ND, not detected.

Article Snippet: A oneway ANOVA and Dunnett’s posttest were used to determine the positive value cutoff (P £ 0.05) for the identification of CLas from infected samples. qPCR assay of CLas nrdB. qPCR was performed with TempAssure 0.1-ml PCR tube strips (USA Scientific) with optical caps in a real-time thermal cycler (Bio-Rad CFX96).

Techniques: CRISPR, Real-time Polymerase Chain Reaction, Recombinase Polymerase Amplification, Negative Control

Journal: Phytopathology®

Article Title: Highly Sensitive and Rapid Detection of Citrus Huanglongbing Pathogen (‘Candidatus Liberibacter asiaticus’) Using Cas12a-Based Methods

doi: 10.1094/phyto-09-20-0443-r

Figure Lengend Snippet: Fig. 5. Detection of ‘Candidatus Liberibacter asiaticus’ (CLas)-infected samples via DNA endonuclease-targeted CRISPR trans reporter (DETECTR) assay. Detection of CLas was performed on 28 DNA samples from sweet orange (orange), pumelo (green), grapefruit (yellow), periwinkle (purple), psyllid (gray). The grapefruit samples GFTN1 and GFTN2 were potentially infected but showed negative for CLas based on quantitative PCR detection. Genomic DNAs extracted from uninfected grapefruit cultivar Duncan (NC1) and CLas-free psyllids (NC2) were used as negative controls. Error bars indicate the mean of the highest fluorescence value over 1 h ± SD when n = 3 replicates. A two-tailed t test was done to provide P values of infected samples compared with control, where *P £ 0.01 and **P £ 0.001.

Article Snippet: A oneway ANOVA and Dunnett’s posttest were used to determine the positive value cutoff (P £ 0.05) for the identification of CLas from infected samples. qPCR assay of CLas nrdB. qPCR was performed with TempAssure 0.1-ml PCR tube strips (USA Scientific) with optical caps in a real-time thermal cycler (Bio-Rad CFX96).

Techniques: Infection, CRISPR, Real-time Polymerase Chain Reaction, Fluorescence, Two Tailed Test, Control